FlipGFP protease assay for evaluating in vitro inhibitory activity against SARS-CoV-2 Mpro and PLpro
Description
FlipGFP assay characterizes the intracellular drug target engagement to M^(pro) and PL^(pro) and can be performed in the biosafety level 1/2 settings. Here, we provide the detailed protocol for the cell-based FlipGFP assay to identify and